reagent sulfo chromalink biotin Search Results


ezlink sulfo-nhs-ss-biotin  (Thermo Fisher)
90
Thermo Fisher ezlink sulfo-nhs-ss-biotin
Ezlink Sulfo Nhs Ss Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ezlink sulfo-nhs-ss-biotin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ezlink sulfo-nhs-ss-biotin - by Bioz Stars, 2026-03
90/100 stars
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sulfo-nhs-ss-biotin  (Thermo Fisher)
90
Thermo Fisher sulfo-nhs-ss-biotin
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Sulfo Nhs Ss Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sulfo-nhs-ss-biotin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
sulfo-nhs-ss-biotin - by Bioz Stars, 2026-03
90/100 stars
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ez-link sulfo-nhs-ss-biotin  (Thermo Fisher)
90
Thermo Fisher ez-link sulfo-nhs-ss-biotin
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Ez Link Sulfo Nhs Ss Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ez-link sulfo-nhs-ss-biotin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ez-link sulfo-nhs-ss-biotin - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ez-link sulfo-nhs-biotin reagent  (Thermo Fisher)
90
Thermo Fisher ez-link sulfo-nhs-biotin reagent
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Ez Link Sulfo Nhs Biotin Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ez-link sulfo-nhs-biotin reagent/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ez-link sulfo-nhs-biotin reagent - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ez-link sulfo-nhs-biotin reagents  (Thermo Fisher)
90
Thermo Fisher ez-link sulfo-nhs-biotin reagents
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Ez Link Sulfo Nhs Biotin Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ez-link sulfo-nhs-biotin reagents/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ez-link sulfo-nhs-biotin reagents - by Bioz Stars, 2026-03
90/100 stars
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ez-link sulfo-nhs (n-hydroxysuccinimido)-biotin  (Thermo Fisher)
90
Thermo Fisher ez-link sulfo-nhs (n-hydroxysuccinimido)-biotin
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Ez Link Sulfo Nhs (N Hydroxysuccinimido) Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ez-link sulfo-nhs (n-hydroxysuccinimido)-biotin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
ez-link sulfo-nhs (n-hydroxysuccinimido)-biotin - by Bioz Stars, 2026-03
90/100 stars
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membrane-impermeable biotinylation reagent ez-link sulfo-nhs-biotin  (Thermo Fisher)
90
Thermo Fisher membrane-impermeable biotinylation reagent ez-link sulfo-nhs-biotin
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Membrane Impermeable Biotinylation Reagent Ez Link Sulfo Nhs Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/membrane-impermeable biotinylation reagent ez-link sulfo-nhs-biotin/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
membrane-impermeable biotinylation reagent ez-link sulfo-nhs-biotin - by Bioz Stars, 2026-03
90/100 stars
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nhs-lc-biotin reagent  (Thermo Fisher)
90
Thermo Fisher nhs-lc-biotin reagent
Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked <t>biotinylation</t> reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.
Nhs Lc Biotin Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nhs-lc-biotin reagent/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
nhs-lc-biotin reagent - by Bioz Stars, 2026-03
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Image Search Results


Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked biotinylation reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.

Journal: The Journal of Biological Chemistry

Article Title: Mechanism and Function of Monoclonal Antibodies Targeting Siglec-15 for Therapeutic Inhibition of Osteoclastic Bone Resorption *

doi: 10.1074/jbc.M113.494542

Figure Lengend Snippet: Antibody-induced internalization and lysosomal degradation of Siglec-15 in osteoclasts. A, internalization of biotinylated Siglec-15. RAW264.7-derived osteoclast cell-surface proteins were labeled with a disulfide-linked biotinylation reagent. Cells were then treated with a combination of primary (anti-Siglec-15 B02 or control IgG) antibodies (at 4 °C) followed by secondary cross-linking antibodies (10 min at 37 °C, lanes 5 and 6), or with primary antibody alone (10 min at 37 °C, lanes 7 and 8). Control cells were incubated with B02, control IgG, or no antibody at 4 °C (lanes 1–3) without a 37 °C chase. After these antibody treatments, remaining cell-surface biotin was stripped with a reducing agent; for one sample, as an additional control (lane 4), this stripping step was omitted. Internalized, biotinylated proteins were collected from cell lysates by streptavidin immunoprecipitation, and Siglec-15 was detected by Western blotting. B, characterization of Siglec-15 endocytosis by confocal microscopy. RAW264.7-derived osteoclasts were cold-loaded with Siglec-15 antibody and either fixed immediately (No chase) or incubated in fresh, warm media for 10 or 45 min prior to fixation. Cells were then permeablized and stained with anti-LAMP2 and anti-human IgG (to detect internalized Siglec-15). C, Siglec-15 protein levels decrease rapidly following antibody treatment. Differentiated RAW264.7-derived osteoclasts were treated for the indicated times with anti-Siglec-15 or a control human IgG. Protein lysates were analyzed by Western blotting with the indicated antibodies. D, RAW264.7 differentiated in the presence of Siglec-15 antibodies show decreased Siglec-15 protein levels. Protein lysates of RAW264.7 cells, either non-differentiated (− RANKL) or differentiated (+ RANKL) in media with or without anti-Siglec-15 were analyzed by Western blotting with the indicated antibodies.

Article Snippet: Biotin Internalization Assay To biotinylate cell-surface proteins, differentiated RAW264.7-derived osteoclasts were rinsed twice with cold PBS containing 1 m m CaCl 2 and 1 m m MgCl 2 (PBS/calcium/magnesium, HyClone) and incubated with the biotinylation reagent sulfo-NHS-SS-biotin (Pierce), diluted to 1 mg/ml in PBS/calcium/magnesium for 1 h at 4 °C.

Techniques: Derivative Assay, Labeling, Incubation, Stripping Membranes, Immunoprecipitation, Western Blot, Confocal Microscopy, Staining